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Testing Kit

89,00

Testing Kit

89,00

Orders made up to 11:59am are usually shipped the same very same business day or within 48 hours. Free shipment for orders above €199! The average delivery time is 2-5 working days (see our shipping page for more info).

38 in stock (can be backordered)

SKU: TT01 Category:
Buy 6 pcs and get 10% off
Buy 3 pcs and get 5% off

Description

The Cannabinoid testing kit (called Alpha-CAT) combines the simple analytical technique of thin-layer chromatography (TLC) with specific thin-layers and developing fluid.

Cannabis Test Finger Print

Thanks to this new protocol the main active ingredients of the cannabis plant parts can be visualised due to a highly specific colouring reaction which represent the cannabinoids present in the sample of cannabis or cannabis product. It enables the detection and identification of six (6) of the main cannabinoids present in cannabis:

  • Cannabidiol (CBD)
  • Cannabinol (CBN)
  • Tetrahydrocannabinol (THC)
  • Tetrahydrocannabivarin (THCV)
  • Cannabigerol (CBG)
  • Cannabichromene (CBC)

A minimal amount of material (0.1 gram) is needed per sample to provide a unique and effective way of analysing the cannabinoid profile in various mixtures such as plant parts of cannabis, trichome resins, oils, tinctures and edibles products (ice-creams, cookies, teas…).

alphacat cannabis test

The Cannabinoid testing kit helps you to assess the effect of any specific strain with:

  • A THC and CBD reference chart for indication of strength and potency
  • Chemotype / cannabinoid profile which categorizes the strain
  • Chemotype-effect chart indicateing the  pharmacological effect to be expected
  • A symptom-cure guide helping to determine which strain best helps to alleviate specific symptoms.

The Cannabinoid testing kit contains:

2 plates

Number of tests*:

– THC & CBD analysis: 8

– Chemotype prints: 2

*Each TLC plate allows for testing 4 lanes. A full analysis uses all 4 lanes for 1 strain. To get a chemotype print of your strain, 1 line is sufficient: 4 strains can therefore be analysed on 1 TLC plate.

THE FOLLOWING SHOULD BE SUPPLIED BY THE USER

  • Samples of cannabis or cannabis products (e.g. cookies, ice, cake, etc.)
  • A well ventilated working area (a fume hood connected to a filtering system is recommended)
  • Digital weighing scale

ALPHA-CAT TESTING PROTOCOL

READ THE PROTOCOL MANUAL

alpha-CAT, the MasterClass by Alchimia Web on Youtube

The Cannabinoid testing kit system solves the issues involved with simultaneously separating  cannabinoids in a simple, cost-effective way, providing a chemical profile for each sample. The method separates some of the neutral and acidic cannabinoids which are rendered distinct from each other with bright colored spots.

The chemotype profile shows the number of cannabinoids and their relative concentrations. The higher the concentration the bigger and more intensely coloured the spot is.The Cannabinoid testing kit gives the opportunity to determine the cannabinoids present in each material sample and reveals the levels of each individual cannabinoid present in terms of its concentration.

The analysis method enables anyone, regardless of scientific background, to define the active cannabinoid profile in a wide range of medicinal materials (flowers, resins, concentrates, cosmetics, tinctures, edibles and most other cannabinoid containing products).

Different types of samples, from cannabinoid herbal based to other cannabinoid derivates (left to right: Bedica®, Bedrocan®, tincture, flower, resin "haschich")

Different types of samples, from cannabinoid herbal based to other cannabinoid derivates (left to right: Bedica®, Bedrocan®, tincture, flower, resin “hashish”)

HOW TO ANALYSE THE RESULTS

After colouring the TLC plate by dipping it in a dye solution colored spots will appear immediately. The number of different spots depends entirely on the plant used (genetics) and the environmental parameters during cultivation. The bigger the spot is, the higher is the concentration.

The combination of different spots is called the chemotype of this particular plant (strain). Samples derived from it, such as the dried flowers, edibles, oils and hashish will differ somewhat in composition and won’t show the same cannabinoid profile (or print). Each colored spot on the plate represents a cannabinoid.

THC Calibration Cannabis test

CBD Calibration Chart & TLC plate results. Use the calibration charts from the kit for proper reference.

The combination of all cannabinoids present in a particular strain will be responsible for the pharmaceutical effects as well as the side-effects commonly reported and will range from an ultimate high feeling/experience (only THC present) to a heavy, “stoney”, “narcotic” sleep provoking strain with low THC when compared to its levels of CBD and CBG or with overall high levels of CBD and CBG. Small differences in cannabinoid levels of THCV, CBG and CBC between strains results in tremendous differences in perceived effect.

In order to translate this into a potency concentration, we need to know the exact weight of our sample.
Since large concentrations will produce larger sized colored spot, we need to reduce the sample weight, so that the spot does not become to large to be measured correctly on the testing plate by a ruler. Once you obtain the size of the colored spots, we need to apply a coefficient called the “Multiplication Factor”. You will need to remember the sample weight (use a scale ranging to 0,001g – 0,01g or 1oz) and the amount of extraction fluid used.
The formula to calculate the Multiplication factor:
Multiplication Factor = (100mg x 2 µI) / (samples weight (mg) x extraction fluid (µI))

Examples

 

Sample weight for concentrated samples (high CBD concentration)
In a case of a concentrate sample when you expect between 40% and 100% CBD, you will take 40mg of sample with 1µl extraction fluid with the suitable capillary tube, using the following multiplication factor: ( 100 x 2 ) / ( 40 x 1 ) = 5 ( multiplication factor )
Then after reading the cannabinoid % on the plate with the ruler, calculate with the Multiplication Factor the true %
e.g. when you read 13% CBD with the ruler then multiply by 5 which will result in 65% CBD.
Sample weight for diluted samples (Low CBD concentration)
In a case of a diluted samples when you expect between 0,2% and 5%
CBD you will take 200 mg of samples with 8 µI extraction fluid with the suitable capillary tube, then: The multiplication factor will be (100 x 2) / (200 x 8) = 0.125 (multiplication factor)
Then after reading the cannabinoid % on the plate with the ruler,
calculate with the multiplication factor the true %, for example when you read 5% CBD with
the ruler then multiply by 0.125 which will result in 0.63% CBD.

SAFETY RECOMMENDATION

  • Alpha-CAT® kits are fully compliant with the guidelines of the World Health Organisation concerning safety and quality of medical plant materials (WHO/PHARM/92.559).
  • Our methods meet the guidelines and recommendations of the Food and Agriculture Organisation (FAO) Codex Alimentarius.
  • All of our kits comply fully with Hazard Analysis Critical Control Points (HACCP) guidelines.

alpha-CAT’s kits contains bottles of chlorinated hydrocarbons, labelled as hazardous and the following precautions should be observed at all times:

Payment

You can make your order through by clicking on the button “Add to Basket”) Fill in your address and you will be provided with our payment instructions for direct bank transfers or payments with PayPal.

If you prefer to make your payment by Credit card, then choose the PayPal option within the checkout section.

Need help?

Chat with us or have a look in our FAQ page!

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